The EPC 10 USB amplifiers are available with one (EPC 10 USB), two (EPC 10 USB Double), three (EPC 10 USB Triple) or four (EPC 10 USB Quadro) amplifiers. This hardware product line is fully integrated with HEKA's data acquisition LIH 8+8 AD/DA interface. The interface utilizes USB 2.0 and high speed processing technologies. The advanced integration of the EPC 10 USB with the interface and software minimizes total recording noise, eliminates compatibility problems and reduces additional equipment expenses and set-up time.
The digital control of all amplifier functions has been designed with two advantages in mind. Firstly, as the computer controls the functions of the amplifier, the data acquisition program can access and store variables that describe all of the settings during an experiment. Secondly, computer control allows the user to highly automated the experiments.
These include automatic switching (e.g, switching between the settings for establishing a seal or those for single channel recording) and also the automatic adjustment of capacitance transients cancellation and series resistance compensation. In fact, digital control of every adjustable parameter in the amplifier circuitry is implemented, including the calibration adjustments.
The EPC 10 USB is a complete data acquisition system, which can be controlled with HEKA's PATCHMASTER or TIDA software. A DLL (dynamic link library) is available to allow access of the system from a custom written application. The EPC 10 USB patch clamp amplifier, combined with a computer and PATCHMASTER or TIDA software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system.
A. S. Axelsson, T. Mahdi, H. A. Nenonen, T. Singh, S. Hänzelmann, A. Wendt, A. Bagge, T. M. Reinbothe, J. Millstein, X. Yang, B. Zhang, E. G. Gusmao, L. Shu, M. Szabat, Y. Tang, J. Wang, S. Salö, L. Eliasson, I. Artner, M. Fex, J. D. Johnson, C. B. Wollheim, J. M. J. Derry, B. Mecham, P. Spégel, H. Mulder, I. G. Costa, E. Zhang and A. H. Rosengren (2017). "Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes." Nature Communications 8: 15652.
S. Bahari-Javan, H. Varbanov, R. Halder, E. Benito, L. Kaurani, S. Burkhardt, H. Anderson-Schmidt, I. Anghelescu, M. Budde, R. M. Stilling, J. Costa, J. Medina, D. E. Dietrich, C. Figge, H. Folkerts, K. Gade, U. Heilbronner, M. Koller, C. Konrad, S. Y. Nussbeck, H. Scherk, C. Spitzer, S. Stierl, J. Stöckel, A. Thiel, M. von Hagen, J. Zimmermann, A. Zitzelsberger, S. Schulz, A. Schmitt, I. Delalle, P. Falkai, T. G. Schulze, A. Dityatev, F. Sananbenesi and A. Fischer (2017). "HDAC1 links early life stress to schizophrenia-like phenotypes." Proceedings of the National Academy of Sciences 114(23): E4686-E4694.
A. Brechet, R. Buchert, J. Schwenk, S. Boudkkazi, G. Zolles, K. Siquier-Pernet, I. Schaber, W. Bildl, A. Saadi, C. Bole-Feysot, P. Nitschke, A. Reis, H. Sticht, N. Al-Sanna´a, A. Rolfs, A. Kulik, U. Schulte, L. Colleaux, R. Abou Jamra and B. Fakler (2017). "AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability." Nature Communications 8: 15910.
S. Cains, C. Blomeley, M. Kollo, R. Rácz and D. Burdakov (2017). "Agrp neuron activity is required for alcohol-induced overeating." Nature Communications 8: 14014.
G. Chen, Y. H. Kim, H. Li, H. Luo, D.-L. Liu, Z.-J. Zhang, M. Lay, W. Chang, Y.-Q. Zhang and R.-R. Ji (2017). "PD-L1 inhibits acute and chronic pain by suppressing nociceptive neuron activity via PD-1." Nature Neuroscience 20(7): 917-926.
C. E. Constantin, C. S. Müller, M. G. Leitner, W. Bildl, U. Schulte, D. Oliver and B. Fakler (2017). "Identification of Cav2-PKCß and Cav2-NOS1 complexes as entities for ultrafast electrochemical coupling." Proceedings of the National Academy of Sciences 114(22): 5702-5712.
J. J. Fink, T. M. Robinson, N. D. Germain, C. L. Sirois, K. A. Bolduc, A. J. Ward, F. Rigo, S. J. Chamberlain and E. S. Levine (2017). "Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells." Nature Communications 8: 15038.
R. Fliegert, A. Bauche, A.-M. Wolf Perez, J. M. Watt, M. D. Rozewitz, R. Winzer, M. Janus, F. Gu, A. Rosche, A. Harneit, M. Flato, C. Moreau, T. Kirchberger, V. Wolters, B. V. L. Potter and A. H. Guse (2017). "2[prime]-Deoxyadenosine 5[prime]-diphosphoribose is an endogenous TRPM2 superagonist." Nature Chemical Biology (advance online publication).
M. Harkat, L. Peverini, A. H. Cerdan, K. Dunning, J. Beudez, A. Martz, N. Calimet, A. Specht, M. Cecchini, T. Chataigneau and T. Grutter (2017). "On the permeation of large organic cations through the pore of ATP-gated P2X receptors." Proceedings of the National Academy of Sciences 114(19): E3786-E3795.
I. J. Jang, A. J. Davies, N. Akimoto, S. K. Back, P. R. Lee, H. S. Na, H. Furue, S. J. Jung, Y. H. Kim and S. B. Oh (2017). "Acute inflammation reveals GABAA receptor-mediated nociception in mouse dorsal root ganglion neurons via PGE2 receptor 4 signaling." Physiological Reports 5(8).
M.-H. Jeong, H.-J. Kim, J.-H. Pyun, K.-S. Choi, D. I. Lee, S. Solhjoo, B. O'Rourke, G. F. Tomaselli, D. S. Jeong, H. Cho and J.-S. Kang (2017). "Cdon deficiency causes cardiac remodeling through hyperactivation of WNT/ß-catenin signaling." Proceedings of the National Academy of Sciences 114(8): E1345-E1354.
S. L. Johnson, J. Olt, S. Cho, H. von Gersdorff and W. Marcotti (2017). "The Coupling between Ca2+ Channels and the Exocytotic Ca2+ Sensor at Hair Cell Ribbon Synapses Varies Tonotopically along the Mature Cochlea." Journal of Neuroscience 37(9): 2471-2484.
C.-H. Liu, Z. Gong, Z.-L. Liang, Z.-X. Liu, F. Yang, Y.-J. Sun, M.-L. Ma, Y.-J. Wang, C.-R. Ji, Y.-H. Wang, M.-J. Wang, F.-A. Cui, A. Lin, W.-S. Zheng, D.-F. He, C.-x. Qu, P. Xiao, C.-Y. Liu, A. R. B. Thomsen, T. Joseph Cahill Iii, A. W. Kahsai, F. Yi, K.-H. Xiao, T. Xue, Z. Zhou, X. Yu and J.-P. Sun (2017). "Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling." Nature Communications 8: 14335.
M. M. Picher, A. Gehrt, S. Meese, A. Ivanovic, F. Predoehl, S. Jung, I. Schrauwen, A. G. Dragonetti, R. Colombo, G. Van Camp, N. Strenzke and T. Moser (2017). "Ca2+-binding protein 2 inhibits Ca2+-channel inactivation in mouse inner hair cells." Proceedings of the National Academy of Sciences 114(9): E1717-E1726.
A. Ramot, Z. Jiang, J.-B. Tian, T. Nahum, Y. Kuperman, N. Justice and A. Chen (2017). "Hypothalamic CRFR1 is essential for HPA axis regulation following chronic stress." Nature Neuroscience 20(3): 385-388.
L. Romani, V. Oikonomou, S. Moretti, R. G. Iannitti, M. C. D'Adamo, V. R. Villella, M. Pariano, L. Sforna, M. Borghi, M. M. Bellet, F. Fallarino, M. T. Pallotta, G. Servillo, E. Ferrari, P. Puccetti, G. Kroemer, M. Pessia, L. Maiuri, A. L. Goldstein and E. Garaci (2017). "Thymosin [alpha]1 represents a potential potent single-molecule-based therapy for cystic fibrosis." Nature Medicine 23(5): 590-600.
N. Romanò, A. Guillou, D. J. Hodson, A. O. Martin and P. Mollard (2017). "Multiple-scale neuroendocrine signals connect brain and pituitary hormone rhythms." Proceedings of the National Academy of Sciences 114(9): 2379-2382.
W. Shen, C. Nan, P. T. Nelson, H. Ripps and M. M. Slaughter (2017). "GABAB receptor attenuation of GABAA currents in neurons of the mammalian central nervous system." Physiological Reports 5(6).
A. Sigler, W. C. Oh, C. Imig, B. Altas, H. Kawabe, B. H. Cooper, H.-B. Kwon, J.-S. Rhee and N. Brose (2017). "Formation and Maintenance of Functional Spines in the Absence of Presynaptic Glutamate Release." Neuron 94(2): 304-311.e304.
P. Somasundaram, G. R. Wyrick, D. C. Fernandez, A. Ghahari, C. M. Pinhal, M. Simmonds Richardson, A. C. Rupp, L. Cui, Z. Wu, R. L. Brown, T. C. Badea, S. Hattar and P. R. Robinson (2017). "C-terminal phosphorylation regulates the kinetics of a subset of melanopsin-mediated behaviors in mice." Proceedings of the National Academy of Sciences 114(10): 2741-2746.
A. Stange-Marten, A. L. Nabel, J. L. Sinclair, M. Fischl, O. Alexandrova, H. Wohlfrom, C. Kopp-Scheinpflug, M. Pecka and B. Grothe (2017). "Input timing for spatial processing is precisely tuned via constant synaptic delays and myelination patterns in the auditory brainstem." Proceedings of the National Academy of Sciences 114(24): E4851-E4858.
L. Tao, L. Peng, R. P. A. Berntsson, S. M. Liu, S. Park, F. Yu, C. Boone, S. Palan, M. Beard, P.-E. Chabrier, P. Stenmark, J. Krupp and M. Dong (2017). "Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors." Nature Communications 8(1): 53.
C. Wetzel, S. Pifferi, C. Picci, C. Gok, D. Hoffmann, K. K. Bali, A. Lampe, L. Lapatsina, R. Fleischer, E. S. J. Smith, V. Begay, M. Moroni, L. Estebanez, J. Kuhnemund, J. Walcher, E. Specker, M. Neuenschwander, J. P. von Kries, V. Haucke, R. Kuner, J. F. A. Poulet, J. Schmoranzer, K. Poole and G. R. Lewin (2017). "Small-molecule inhibition of STOML3 oligomerization reverses pathological mechanical hypersensitivity." Nature Neuroscience 20(2): 209-218.
Wu, Z., N. Grillet, B. Zhao, C. Cunningham, S. Harkins-Perry, B. Coste, S. Ranade, N. Zebarjadi, M. Beurg, R. Fettiplace, A. Patapoutian and U. Muller (2017). "Mechanosensory hair cells express two molecularly distinct mechanotransduction channels." Nature Neuroscience 20(1): 24-33.
A. J. Yiu, C.-L. Ibeh, S. K. Roy and B. C. Bandyopadhyay (2017). "Melamine induces Ca2+-sensing receptor activation and elicits apoptosis in proximal tubular cells." American Journal of Physiology - Cell Physiology 313(1): C27-C41.
X. Zhang and A. N. van den Pol (2017). "Rapid binge-like eating and body weight gain driven by zona incerta GABA neuron activation." Science 356(6340): 853-859.
L. Zhou, J. McInnes, K. Wierda, M. Holt, A. G. Herrmann, R. J. Jackson, Y.-C. Wang, J. Swerts, J. Beyens, K. Miskiewicz, S. Vilain, I. Dewachter, D. Moechars, B. De Strooper, T. L. Spires-Jones, J. De Wit and P. Verstreken (2017). "Tau association with synaptic vesicles causes presynaptic dysfunction." Nature Communications 8: 15295.
Order Number: 895000EPC 10 USB Single Patch Clamp Amplifier including 1x Red Star Headstage, 1x Pipette Holder (1.5 mm), 1x Model Cell (MC-10)
Order Number: 895255EPC 10 USB Single Main Unit (no headstage, no pipette holder included)
Order Number: 895273EPC 10 USB Single Patch Clamp Amplifier including 1x Red Star Headstage, 1x Pipette Holder (1.5 mm), 1x Model Cell (MC-10), 1 Patchmaster Software License
Order Number: 895277EPC 10 USB Single Patch Clamp Amplifier including 1x S-Probe Headstage, 1x Pipette Holder (1.5 mm), 1x Model Cell (MC-10), 1 Patchmaster Software License
The EPC 10 USB Patch Clamp Amplifiers are shipped by default with 1.5 mm (OD) pipette holders. Other diameters are available on request.